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生物物理学报 1991
INVESTIGATION OF HYPOCRELLIN B SENSITIZED PHOTODAMAGE TO THE FLUIDITY OF ERYTHROCYTE MEMBRANE
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Abstract:
Three static fluorescence probes: 1, 6-diphenyl-1, 3, 5-hexatriene (DPH), l-anilino-8-naphthalene sulffonate (ANS) and pyrene were used to study the fluidity of erythrocyte membrane.We found that the degree of polarization increased, after visible light illumination in the presence of Hypocrellin B. It means that the membrane fluidity and the lateral diffusion rate decreased (by measuring pyrene excimer/monomer ratio) with increasing concentration of hypocrellin B.To elucidate the mechanism of photodamage of hypocrellin B in biological systems, 1O2 quenchers such as, 1, 4-diazabicyclo (2, 2, 2) octane; L-histidine; OH- radical quencher: N-(p-AminoBenzoyl) -L-Glutamic Acid(PABGA); antioxi-dant: Butylated hydroxytoluene (BHT)reagent and Deuterium solvent effects were investigated. The results showed that the photodamages to fluidity of erythrocyte membranes were not only due to the simple mechanism of 1O2; but also due to other radicals as well. We also compared the effect of different concentrations of HB and HA on the polarizability of erythrocyte membranes it was found that the effect of HB was greater than that of HA when the concentrations were more that 4×10-8M.
