Fusion Expression of a Peptide Antibiotic-apidaecin Gene in Lactococcus lactis
多肽抗生素apidaecin基因在乳酸乳球菌中的融合表达

The ubiquitin fusion of apidaecin was expressed in Lactococcus lactis ,using a novel nisin inducible expression system.After induction,a specific band could be detected in the extracts of the host strain by Tricine SDS PAGE and Western blotting. Production of the fusion was up to 7 2% of the total soluble protein of the host strain.While the fusion was cut by ubiquitin specific protease\|UBP1,the product had distinct antibacterial activity.