Expression of Human Hepatocyte Growth Factor with Chinese Hamster Ovary(CHO) Cells
人肝细胞生长因子cDNA克隆在CHO细胞中的表达

The cDNA gene encoding human hepatocyte growth factor(hHGF)was inserted into pcDNA3 plasmid and obtained pcDNA3/hHGF expression plasmid.The pcDNA3/hHGF plasmid was transfected into CHO cells with calcium phosphate DNA precipitate method.The expression clones was obtained by G418 selection.The culture supernatant from the positive clones could stimulating the mitosis of primary culture of rat hepatocytes and inhibiting proliferation of HepG2 cells were observed.