Expression of Calmodulin binding Domain of Neuronal Nitric Synthase and Its Binding Activity to Calmodulin
脑型一氧化氮合成酶的钙调蛋白结合区的表达及活性鉴定

To facilitate to study the associations of nNOS functions with its calmodulin(CaM) binding domain and to prepare nNOS specific inhibiting peptides from phage peptide library,we have amplified the coding gene of nNOS CaM binding domain(nNOS2544 2988bp) and expressed it in E.coli .The recombinant product in size of 22kDa was purified (over 90% in pure) by His.Tag Sephorose column and its obvious CaM binding activity was detected with CaM overlay assay.Since the sequence specificity and effective calmodulin binding activity,the protein was considered as an ideal target for screening nNOS specific peptides from peptide library and also an antigen for raising nNOS antibody.