THE EFFECTS OF ACETYLATION ON STRUCTURE OF PURPLE MEMBRANE
酰化对紫膜结构的影响

The acetylation-induced changes in spectra with respect to UV/VIS absorption, RAMAN and Flash Kinetic Spectrum of purple membrane were determined in a wide range of wavelength and PH. It was found that the change of surface potential of the purple membrane resulting from acetylation induced the change of local protein conformation Which could be delocalized all over the protein to a great extent and affected the nature of binding sites of retinal and the structure of the retinal chromophore. Therefore, measurable spectral changes occurred, The acetylated purple membrane became less stable to pH perturbation than the unacetylated membrane.,