Construction of a YAC Contig Encompassing G200 Locus via Chromosome Walking
通过染色体步查建立水稻G200座位的重叠群

A YAC contig was constructed by screening a rice genomic YAC library with G200,Y2587L,Y4073L and L688 RFLP markers as probes.4 positive clones with the sizes ranged from 240kb to 650kb were obtained.The end fragments of YAC inserts were isolated via inverse PCR,with whom a YAC contig of 3cM was initially established.Chromosome walking was performed with the two distal end fragments of the YAC contig and some positive YAC clones were obtained and identified. Thus the G200 contig was extended to about 8cM.