The Study on Integration and Expression of Porcine Growth Hormone Genein Transgenic Goldfish
外源猪生长激素基因在金鱼体内的整合与表达研究

A linear recombinant plasmid, consisting of sheep metallothionein gene promoter and porcine growth hormone gene, was microinjected into goldfish fertilized eggs. The experimental goldfishes were detected and screened by using dot blotting and Southern blotting to obtain foreig gene integration positive transgenic goldfish, which were further used for mating each other. F1 and F2 generations were detected by using PCR, respectively. Our data, including PGH expression level in transgenic goldfish serum detected radioimmunoassy, showed that foreign genes have been integrated and expressed in some transgenic goldfish, and passed into next generation through sex reproduction.