Studies on Synthesis of Glutathione by E. coliBL21 (pTrc-gsh) Coupled with Saccharomyces cerevisiae
大肠杆菌BL21(pTrc-gsh)与酵母耦联合成谷胱甘肽的研究

During synthesis of GSH by the engineered strain E.coli BL21(pTrc-gsh) coupled with Saccharomyces cerevisiae producing ATP from adenosin,the inconsistency of two systems in the concentration of phosphate buffer was solved by decreasing concentration to 250mmol/L.The conditions under 250mmol/L phosphate buffer were optimized and the yield of GSH was 1.6g/L,which was higher than that of summation by two systems under the same conditions respectively.Addition of glycine later after glutamate and cysteine weakened the inhibition of GSH to GSHI.It made the yield of GSH reach to 2.13g/L which was 30.7% higher than the control.