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OALib Journal期刊
ISSN: 2333-9721
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APPLICATION OF A CARBOCYANINE FLUORESCENT DYE TO TRACING NEURONAL CONNECTIONS OF THE PIGEON TECTUM WITH SOME MESENCEPHALIC NUCLEI
用羰花青荧光染料追踪家鸽顶盖与中脑核团的神经连接

Keywords: Pigeon,Mesencephalon,Tracing methods,Carbocyanine fluorescent dye,Intramembranous diffusion
,中脑,dil荧光染料,膜内扩散

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Abstract:

In this study, diI (1, 1, dioctadecyl-3, 3, 3', 3'-tetramethyl-indocarbocyanine perchlorate) as a derivative of the carbocyanine dye, a fluorescent lipophilic substance, was applied in fixed tissue for tracing the neuronal connections of the deep layers of the tectum with some mesencephalic nuclei in pigeons (Columba livia). The results indicate that the deep layers (II h - III ) of the tectum receive their neuronal inputs from the nucleus isthmi pars magnocellularis (Imc), the nucleus nervi oculomotorii, pars ventralis (OMv) and the nucleus mesencephalicus lateralis, pars dorsalis (MLd). A comparative analysis has been earned out between the results of this study and those obtained with CB-HRP.

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