The Manipulation and Modification of Fruit Ripening by Expression of ACC Deaminase Gene in Tomato
通过表达ACC脱氨酶基因控制番茄果实的成熟

The gene encoding ACC deaminase was cloned by PCR and introduced into tomato plants by Agrobacterium mediated transformation.The Southern and Western blot analysis demonstrated that the gene has been integrated into tomato genome and expressed.These's a correlation between the degree of ethylene inhibition and delay in the process of fruit ripening in transgenic tomato fruits.Fruits from these plants exhibited significant delays in ripening and keep fresh for at least 2 months in vitro. In the line best expressing ACC deaminase gene,synthesis of ethylene in plants was reduced by 80%.