EVALUATION OF IN VITRO STABILITY OF SMALL UNILAMELLAR VESICLES COATED WITH COLLAGEN AND CHITOSAN
胶原、壳聚糖包覆的小单层脂质体的体外稳定性评价

To improve the stability of small unilamellar vesicles(SUV) in vitro, the permeability of SUV from soybean phosphatidylcholine (PC) coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein(5(6)-CF) as a fluorescence probe. Results showed that coating with collagen/PC weight ratio of 2 or chitosan/PC weight ratio of 8 decreased significantly the permeability of the liposomal membrane. Moreover, fluorescence polarization method was used to study the influence of coating with collagen and chitosan at the above ratio to PC on the fluidity of the liposomal membrane, by using an intramolecular charge transfer compound 3-methoxy-4'-N, N-dimethylamino flavone derivative(DMMF) as a fluorescence probe. Results showed that the fluidity of the liposomal membrane after coating with the two macromolecules had no obvious changes. It indicates that SUV coated with collagen and chitosan at appropriate weight ratio can significantly improve the stability of the liposomal membrane in vitro without disturbing its fluidity.