Nuclear Transplantation Between Animals of Different Classes__the Combination of the Nucleus of Mouse and the Cytoplasm of Loach
不同纲动物间的细胞核移植——将小鼠细胞核移到泥鳅细胞质中

The nucleus of a blastula cell from mouse(mus musculus albula,class mammalia,2N=40) was transplanted into an enucleated egg of loach (Paramisgurnus dabryanus,class pisces,2N=48). Of 79 nucleo cytoplasmic hybrid (NCH) eggs,20(25.3%)developed to blastula,but no gastrula was obtained.Developmental incompatibility between donor nucleus and recipient cytoplasm existed,which was expressed by the formation of multispindles.The multispindle was proposed to result in chromosome exclusion in the NCH egg.Such chromosome exclusion might be an important reason for the abnormal development of NCH egg and the cessation of the further development of NCH blastula in this mouse donor and loach recipient combination.