RADIOSENSITIZATION OF CHINESE HAMSTER V79 CELLS BY A NITROTRIAZOLE DERIVATIVE, TA-101, IN VITRO
Nitrotriazole(NTA)衍生物对中国仓鼠V_(79)细胞的辐射增敏作用

TA-101 is a hydrophilic amide derivative of nitrotriazole. The sensitizing activities were evaluated using Chinese hamster V79 cells by conventional colony formation method and comparaed with that of MISO which was kindly supplied by proffesor Adams. The irradiation was performed using a 60Co-r ray source at a dose rate of 0.936 GY/min. The enhancement ratio (ER) was calculated from D37 of survival curves with and without a sensitizer. In our experiment, oxygen enhancement ratio (OER) was 2.5. The control plating efficiencies (PE) were 80±5%. The ERs of TA-101 at the concentration of 0.2, 1.0 and 2.0 mM were 1.49, 2.05 and 2.3 respectively. The result indicates that TA-101 does not have any cytotoxicity to aeroted cells at 0.2mM. A analogue of TA-101 also exhibits no significont cytotoxicity even at 5 mM. Therefore, TA-101 is a potentially efficient radiosensitizer for hypoxic cell in vitro.