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Molecular Cloning and Sequence Analysis of Snowdrop (Galanthus nivalis) Lectin Gene
雪花莲外源凝集素基因的克隆及序列分析

Keywords: Snowdrop (Galanthus nivalis L,)lectin,molecular cloning,sequence analysis

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Abstract:

Snowdrop lectin genes were cloned from DNA extracted from snowdrop young leaves by PCR. Sequence analysis revealed that the cloned gene GNA12 (Galan-thus nivalis agglutinin) which encode mature lectin and GNA34 which encode precursor lectin have a high homology with previously reported LECGNA2 gene. The sequence coding for mature lectin showed 97% and 98% homology at nucleotide and amino acid level respectively. However a 9-base direct repeat in the sequence coding for signal pep-tide of GNA34 makes it different from other previously reported LECGNA genes.

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