Expression of Transthyretin Gene in Yeast Pichia pastoris
转甲状腺素蛋白基因在酵母中的表达

Plasmid pSK TTR was digested by Bam HI and the DNA fragment containing TTR gene (Transthyretin) was cloned into the Bam HI site of secretion vector pHIL SI TTR.In right orientation and frame.After the recombinant plasmid pHIL SI TTR was digested by Bgl II,the larger fragment of it was transformed into yeast Pichia pastoris. The SDS PAGE analysis showed that the yeast transformants could express and secret TTRF.The pure TTRF could be obtained by DEAE Sepherose F.F.chromatography and SephacrylS 200 chromatography.The vitro test showed inhibition of the growth of hepatoma cells by TTRF obtained from our experiments.