Introduction of Wide Spectrum Rice Bacterial Blight Resistance Gene Xa21 into Two-line Genic Male Sterile Rice Variety Pei''''ai 64S
水稻白叶枯病广谱抗性基因Xa21导入两用不育系培矮64S

Agrobacterium mediated transformation of two line genic male sterile Indica rice variety Pei'ai 64S was conducted using a cloned gene,Xa21,as the foreign gene and mature embryo calli as the recipients.A total of 46 transgenic plants had been obtained.The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants.Results of inoculation with philippine race 6 of Xanthomonas oryzae pv.oryzae indicated that most of transgenic plants obtained high resistance to rice bacterial blight disease (Xoo).Analyses of T 1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3:1 ratio.