Studies on Lipase-catalyzed Synthesis of L-ascorbyl Palmitate in Non-aqueous Phase
非水相脂肪酶催化合成L-抗坏血酸棕榈酸酯的研究Ⅰ

The investigation on the reaction media and lipases (NOVO435, MML, LIPOLASE, PPL) for enzymatic synthesis of L-ascorbyl palmitate as well as the factors effecting initial rate of synthesis reaction (rotation speed, temperature, water content, enzyme concentration, and substrate concentration) is presented. Among these investigated solvents and lipases, amyl alcohol and NOVO435 is the optimum pair. The reaction conditions have been optimized: 200 r/min, 55 degrees C, water content = 0, the amount of enzyme = 12.5% of the substrate's quantity.