THE EFFECT OF FUSOGENS ON THE FLUIDITY OF PS LIPOSOMES AND HUMAN ERYTHROCYTE MEMBRANE
膜融合剂对脂质体及血影膜膜流动性的影响

The effect of three fusogens of sucrose, glycerol monooleate and dimethy sulfoxide on the fluidity of PS liposomes and human erythrocyte membrane was investigated by measuring the fluorescence polarization of fluorescent probes of ANS, DPH and AS. The results show that sucrose decreases the fluidity of liposomes and ghost membrane. The more inner is the probe in the hydrophobic region of lipid bilayer, the smaller the decrement is. While GMO increases the fluidity of liposomes and ghost membrane, the more inner is the probe in the hydrophobic region of lipid bilayer, the larger the increment is. DMSO which is at the concentration below 30v/v% increased the fluidity of polar region of ghost membrane labeled on ANS. The effect of DMSO on the fluidity of ghost membrane labeled on DPH appeared two phases. DMSO which at the concentration below 40v/v% decreased the fluidity of ghost membrane, whereas at the concentration above 40v/v%, DMSO increased the fluidity of ghost membrane. The mechanisms of the fluidity changes and the relationship between the changes and chemical - induced membrane fusion have been discussed.