Characterization of Acetylcholinesterase Gene from Insecticide resistant Housefile ( Musca domestica )
杀虫药剂抗性家蝇品系乙酰胆碱酯酶基因的特征分析

Acetylcholinesterase (AChE) is the target site for the organophosphates and carbamates in insects.Widespread use of these two classes of insecticides has led to the selection of resistance.Target modification was regarded as a molecular mechanism in some resistance specics.The altered AChEs with reduced sensitive to inhibition are related to this resistance.AChE genes from two insecticide resistant housefily ( Musca domeslica ) strains D3 and Kash were isolated and sequenced using RT-PCR and streptavidin linked magnetic bead techniques.The cDNAs have a 2082 bp open reading frame from which the complete amino acid sequence of AChE has been deduced .Some differences of nucleotide sequence and four point mutations of amino acid were found compared with that of a susceptible strain Cooper.Three substitutions are likely to confer insecticide insensitivity,which seems that D3 and Kash belong to CII2 pattern of resistance.