Study on Bovine Serum Albumin Recovery from Reversed Micellar Solutions
从反胶束溶液中反萃牛血清白蛋白的研究

From near-infrared spectra, it was confirmed that CTAB/hexanol-octane reversed micellar solution is a suitable medium for bovine serum albumin (BSA) solubiliza-tion. The effects of various factors, such as pH, the type and concentration of salt in stripping solution, on BSA back extraction were studied. The suitable conditions for stripping have been ascertained,i, e. , 1. 0-2. 0mol/L KBr,pH = 4. 3-4. 9. Spectroscopic studies showed that no significant change is observed in the structure of the main chain of BSA after extraction and back extraction. In addition, the problem how long the reversed micellar solution can be recycled have been examined. Finally, the recovery and concentration of protein were successfully obtained with suitable phase ratio.