Recovering PHB from Recombinant Eschenrichia coli by a Combined SDS NaClO Treatment
用SDS．NaClO从重组大肠杆菌中分离聚-β-羟基丁酸酯

A combined SDS NaClO method to recover PHB from recombinant E.coli HMS 174(pTZ18u PHB) was studied.The adding order of SDS and NaClO affected the result of this method and the reason was analyzed.A new process that the cell was treated by SDS for 10min first and then by NaClO for 1min was proposed.Under the conditions of 10g/L biomass,1g/L SDS,and 3%( V/V ) NaClO,the purity of PHB could be increased from 70.87% to 99.3%,the yield was 89%,and the molecular weight(MW) and polydisperity index(pI)of PHB was 1 108 000 and 1.648,resperctively (the initial MW=1 300 000,pI=1.29).