Expression of Trichosanthin in Saccharomyces cerevisiae
成熟天花粉蛋白基因在酵母中的表达

The gene coding for Trichosanthin, a Chinese herb protein was fused to the yeast a factor promoter and its leader sequence. The chimeric gene was constructed in the yeast shuttle plasmid pMM. After transformation of yeast cells with this vector. It was cultured for 24 hours. The highest level of Trichosanthin expressen was detected by anti-Trichosanthin antiserum. The expressed Trichsanthin was found contained in the cell. This is the first report on expression of Trichosanthin in Saccharomyces cerevisiae in the world.