The Improvement of PAS-ELISA for Detection of CMV-free Germplasm in Larger Scale Micropropagation of Banana Plants
检测香蕉花叶心腐病病原CMV的PAS—ELISA方法的改进

Owing to. the application of a routine ELISA test for detection of CMV-free germplasm in larger scale production of the micropropagated banana plants,we found severe false positive interference which caused high rate of germplasm loss. A modified test was developed by addition of sulphite in the solution for preparation of antigen. Statistical data showed that serious interference occured in the samples of the banana sucker and the first 1 - 2 generation of the micropropagated plantlet by using routine technique, and the false positive reaction were almost completely eliminated with the modified method. The dilution curve of purified CMV and TMV verified that there is no difference between the routine and modified methods in term of the sensitivity.