Studies on Three Maize Gene Transfer Techniques
几种玉米基因转移技术的研究及转基因植株的获得

Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries injected after 10-20h of pollination. The results of Dot blotting and Southern blotting analysis certified the integration of Bt gene into maize genome.