Plant Regeneration of Wheat Protoplasts Prepared from Different Composition of Suspension Culture
小麦胚性悬浮系与原生质体植株再生

Regenerable embryogenic cell suspensions initiated from immature embryo-derived 'mix-type' calli of winter wheat (Triticum aestivum L. ) Chang-le 5 served as sources of protoplast. Cell suspension consisted of small calli (2 -3mm in size) and cell clumps (0. 2 -mm in size). The regeneration frequency of the protoplasts derived from small calli of the suspension was remarkably higher than that of cell clumps. The effect of different composition in culture medium including macro elements, vitamins, glycine, glutamin and casein hydrolysate on protoplast division was also investigated.