Metal induced Expression of Mouse Metallothionein 1 cDNAin Synechocystis sp.PCC 6803
小鼠金属硫蛋白在聚胞藻中的金属诱导表达与纯化

Cyanobacterium MT like promoter( smt O P) was used in expression of mMT 1 cDNA in Synechocystis sp.PCC 6803 to enhance metalbinding ability and specialty.Shuttle expression vector pKT MRE was constructed in E.coli (HB101),and through triparental conjugated transfer,Streptomycin screening,Southern and Western blotting analysis,transgenic cyanobacterium clone was acquired.The cultivation and Zn 2 inducing of transgenic cyanobacterium were carried on ,and the foreign mMT 1 was purified through the improved gel filtration and ion exchange chromatography.About 3.5mg mMT 1 were obtained from 5g wet cyanobacterial cells in 5L culture scale.Cyanobacterial metal resistance measurement indicated that the expression of foreign mMT 1 enhanced the transgenic cyanobacterial metalresistance to two times than the wild Synechocystis sp.PCC 6803