Studies on the Metabolic Regulation of Biosynthesis of Lincomycin by Streptomyces lincolnensis
林肯链霉菌合成林可霉素代谢调节的研究

Effects of glucose,ammonium and phosphate on cell growth and lincomycin biosynthesis in fermentation complex medium containing different levels 0.8% of glucose,ammonium or phophate were studied in batch cultures of Streptomyces lincolnensis. In these fermentations the antibiotic biosynthesis was maximal during growth and always declined at the end of the growth phase.In the experiment it was noticed that the final titers of lincomycin were not inhibited by glucose at concentrations of up to 3%.It was found that both phosphate and ammonium salts,while required for cell growth,had negative effects on antibiotic production.High levels 0.8% of ammonium and phosphate inhibit lincomycin formation and increase mycelia growth.During the course of fermentation addition of ammonium or phosphate to the medium at any time led to an increase in mycelial growth.In addition,no inhibition on lincomycin biosynthesis was observed when the supplement of ammonium or phosphate was made during the idiophase,i.e.after 48 houres of fermentation,but not at 24 hours.The earlier the addition of ammonium or phosphate,the more severe the inhibitory effect was.