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生物工程学报 1995
A New Hybrid Obtained by Electrofusion of Protoplasts Between Pleurotus sajor-caju and Pleurotus rhodophyllus
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Abstract:
Some interspecific fusants which successfully was obtained between protoplasts of P. sajor-caju and P. rhodophyllus by electrofusion with BAEKON 2 000 Advanced Gene Transfer System (BAEKON, INE USA). P. sajor-caju and P. rhodophyllus were dikaryons strains with natural genetic markers and used as original parents. The basidiospores of their auxotrophic mutants were used as parent strains. Only fusant(F57) has produced fruit bodies. Based on the characterization of morphology, physiology, Biochemistry and genetics of fusant, F57,and comparision with their parents F57 is a new interspecific hybrid strain.