Research on Hydrogen Production from Organic Compounds with Immobilized Cells of Photosynthetic Bacteria (PSB)
固定化光合细菌利用有机物产氢的研究

The characteristics of hydrogen photoproduction from organic compounds by immobilized Rhodopseudomonas capsulata 386.were studied in batch culture. On the condition that free cells hydrogen-producing cultures were cultured in light for 120 hours, hydrogen production and specific H2 production rate were 68.2ml and 104. 1ml H2/g ?h, respectively. The activity was largely increased when immobilized cells by agar gels was used with hydrogen production up to 128. 4ml and specific hydrogen production rate up to 195. 8ml H2/g ?h. It was proved that in addition to malate. this bacterium could also used glucose, lactate and propionate as hydrogen production substrate and only at appropriate substrate concentration it reached a higher conversion effciency of substrate to H2. Age of inoculum, pH value, light intensity, light dark time and temperature all have different effect patrns.