MSAP analysis of DNA methylation in Arabidopsis (Arabidopsis thaliana) under Oxytetracycline Stress
土霉素胁迫下拟南芥基因组DNA甲基化的MSAP分析

The objectives of this research were to assess the effect of oxytetracycline stress on the development as well as genomic DNA methylation levels and patterns of Arabidopsis thaliana seedlings. Oxytetracycline at concentrations of 3, 5, 7, and 9 mol/L visibly inhibited root and stem enlongation of A. thaliana seedlings, but significantly promoted lateral root proliferation. Methylation sensitive amplified polymorphism (MSAP) analysis showed that genomic DNA methylation levels of oxytetracycline stressed seedlings were lower than that of the untreated plants. The results indicated that oxytetracycline stress induced epigenetic variations in A. thaliana seedlings; no clear trend or pattern of 5-methylcytosine percentage change emerged. At oxytetracycline concentrations of 3, 5, 7, and 9 mol/L, methylation and demethylation of A. Thaliana seedling genomic DNA were respectively 13.29%, 9.22%, 8.03%, 12.59% and 2.80%, 4.26%, 5.11%, 4.90%. We suggest that DNA methylation might be one of the mechanisms used by plants to combat oxytetracycline stress.