Effects of allelochemicals on growth of Bacillus subtilis and its denitrification under anaerobic condition
化感物质对枯草芽孢杆菌(Bacillus subtilis)在厌氧条件下的生长及反硝化作用的影响

The effects of allelochmicals,such as trans\|ferulic acid(t\|FA),phydroxbenzoic acid(p\|HA)and benzoic acid(BA),isolated from decomposed wheat straw on the growth of Bacillus subtilis and its denitrification were investigated.The growth and denitrification of Bacillus subtilis was significantly inhibited by t\|FA at the concentrations of 5.149mmol/L,2.575mmol/L and 0.257mmol/L respectively.However,the effects of p HA at concentrations of 0.362,3.620,7.240mmol/L on growth of Bacillus subtilis were not observed.The growth of B.subtilis was stimulated by BA at concentrations of 4.09 and 8 189mmol/L. The denitrification of B.subtilis was inhibited by both of p HA and BA.The sequence of their inhibition was t\|FA>p\|HA>BA.The inhibition of allelochemicals at a higher concentration was stronger than that at the lower one.Emission of N 2O was inhibited by the above three allelochemicals.The effects of inhibition of allelochemicals in the field remain to be investigated.