Comparison of several important isoenzymes between Bt cotton and regular cotton
转Bt基因抗虫棉与常规棉中几种同工酶的比较——转基因植物安全性评价生理指标初探

The transgenic cotton expressing Bacillus thuringiensis (Bt)insecticidal crystal protein is being commercialized in a large scale in China.Several important isoenzymes,including Peroxidases(POD),Polyphenoloxidases(PPO)and Esterases(EST),were compared between two Bt cotton lines and regular cotton.The activity of Peroxidase in Bt cotton was higher than that in regular cotton.There were also diferences between Bt cotton and regular cotton in the Electrophoretic Patterns of Peroxidase and the activity of Esterase isoenzyme.These physiological differences,maybe caused by genetic manipulation and tissue culture,could induce changes in secondary metabolism of Bt cotton and then bring ecological effects to environment.This kind of effect is also an important aspect of ecological risk assessment of transgenic plants released into environment.