Microsatellite primer-PCR studies on the population differentiation of Aphis gossypii in relation to host plants and seasons
棉蚜(Aphis gossypii)种群寄主分化和季节分化的微卫星引物PCR研究

The present study investigated the differentiation of Aphis gossypii in relation to host plants and seasons This analysis, based on field investigation, lineage breeding and microsatellite primer PCR, showed that populations of A gossypii from three overwinter host plant species, Hibiscus Hibiscus syriacus , Pomegranate Punica granatum and Chinese prickly ash Zanthoxylum simulans , were closely related to a pair wise similarity index ranging from 0 833 to 0 917, suggesting a low level of differentiation among them But a high level of differentiation was found between the populations from overwinter hosts and summer hosts, cotton Gossypium hirsutum and pumpkin Cucurbita pepo , pair wise similarity index ranging from 0 589 to 0 756 The field population of A gossypii may be consist of two genetically determined body colour forms, i e unchangeable yellow form and changeable green form The former is always yellow both in fundatrix and its parthenogenetic offsprings, but in the latter, green fundatrix can produce both green and yellow offsprings The dwarf forms of A gossypii on cotton in summer may come from both green fundatrix and yellow one, but the majority of them may come from the former