Enzyme-linked Immunosorbent Assay Used to Explore the Predation of Chironomus sp. (Diptera:Chironomide) by Predators in Paddy Fields
用ELISA方法检测稻田捕食者对摇蚊的捕食作用

The enzyme-linked immunosorbent assay (ELISA) of double antibody sandwich was used to detect the predation of %Chironomus% sp.by 19 species of predators found in paddy fields. Antiserum was produced in a white male rabbit with an antigen from Chironomus sp., and its high specificity to Chironomus sp. was examined through crossing reaction, neutralization reaction and two-way agar diffusion in laboratory. The thresholds of 19 species of predators to Chironomus sp.were determined. 13 species of predators fed on Chironomus sp., which accounted for 67^89% among the accumulative 19 species tested. Clubiona japonocola collected at early stage of early rice field showed the highest frequency of positive reaction to Chironomus sp. with the frequency of 50%, and 40% frequency of positive reaction to Chironomus sp. was derived from another species, Pirata subpiraticu captured at middle stage of late rice. ELISA would enable the rapid detection of the feeding of predator to objectively reveal the relationships between preys and predators,and evaluate the contribution of predatory arthropods such as spiders to the control of rice pests,so ELISA was suggested to be a valuable tool to aid in developing theories of the community ecology and the integrated rice pest management.