Isolation and phylogenetic study of Tricholoma spp. isolated from Yajiang, Sichuan, China
四川省雅江松茸菌的分离与系统发育

The culturing condition for isolating ecotomycorrhizal fungus Tricholoma spp. was performed, and 10 matsutake isolates were got from Yajiang, Sichuan Province. Using NS1 and NS6, NS1 and NS8, ITS4 and ITS5 primer pairs, the 18S rDNA and ITS fragments of these matsutake strains was amplified, and digested by AluI, HaeIII, HinfI and MspI, respectively. The results showed that these strains had the same restriction patterns for each restriction enzyme, which showed that the homology among these strains were very high. Furthermore, the ITS sequence of representative strain E7 was analyzed, and compared with the data in the Genebank, and then the phylogenic tree was constructed. The results showed that the ITS sequence of E7 was the same as that of T. matsutake KBF20T07 which was isolated from South Korea, and the ITS sequence of E7 had very high similarity to those of the most other matsutake strains in the Genebank. The phylogenetic analysis showed that E7 and the matsutake strains from Genebank were in the same phylogenic branch, which suggested that, from the taxonomic point of view, these matsutake strains isolated from Yajiang belonged to Tricholoma matsutake.