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水生生物学报 2009
The changes of five immune enzymes from Anodonta woodiana infected with Aeromonas hydrophila
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Abstract:
It is suggested that the status and function in the internal defence mechanisms on immune enzymes in seashell have been noticed by more and more scholars in the world at present. Understanding the activation or effect factors of the enzymatic system can enable the evaluation of the health of breed aquatic. The activation of the enzymatic system by bacte-rial or fungal components is an additional clue of the involvement of these enzymes in invertebrate immune defence mecha-nisms. This paper reported the activities of selected enzymes in haemolymph and hepatopancreas from Anodonta woodiana infected by Aeromonas hydrophila. Healthy Anodonta woodiana were collected from Yangzi Island near Poyang Lake, with body length of 13.58±1.12 cm and height of 8.34±0.91cm. Haemolymph and hepatopancreas were extracted at 3h, 6h, 12h, 24h and 48h from the Anodonta woodiana injected with Aeromonas hydrophila(1.6×108cell/mL) and 0.9 % NaC1 solution (control group) respectively. The levels of Superoxide dismutase (SOD), Phenoloxidase (PO), A1 kaline phosphatase (AKP), Acid phosphatase (ACP) and Catalase (CAT) were tested. The results showed that the activities of SOD from haemolymph injected by Aeromonas hydrophila could be significantly higher than that in control at 3h, 12h and 48h respectively, but only at 48h from hepatopancreas. PO activities in serum were notable higher than that in control at 3h and 6h and had the highest level at 3h after injection, then decreased appreciably, however, PO activities in hepato-pancreas increased significantly at 3h. It proved that the activities of SOD and PO in the haemolymph and hepatopancreas of Anodonta woodiana could be induced significantly elevation by Aeromonas hydrophila. While there was no overt differ-ence between injection and control group for the changes of ACP activities, which might indicate that the effect of stimula-tion on Anodonta woodiana with Aeromonas hydrophila was not obvious, so that, it might not stimulate acute increase and release of lysosomes. The same explanation was fit for the change of AKP activities which increased slightly the whole time in the haemolymph and significantly in the hepatopancreas at 3h. In addition, the changes of CAT activities in the haemo-lymph of Anodonta woodiana were not obvious even it restrained notably in the hepatopancreas at 24h and 48h, the enzy-matic responses were compared with other studies and the possible reasons were discussed. It showed that activities of SOD and PO may be used as an indicator on immune disease-resistance, while further research will be needed to understand the activities, functions and mechanism of AKP, ACP and CAT.
