Kinship analysis in the Chinese shrimp (Fenneropenaeus chinensis) base on microsatellite DNA markers
应用微卫星标记分析野生中国明对虾的亲权关系

The mating mode of Chinese shrimp (F.chinensis) is supposed to be pair mating from observation of their mating habits.However,this suggestion has not been confirmed genetically yet.In this study,five microsatellite loci (Fc04,Fc06,Fc18,Fc24 and Fc27) were used to survey the mating mode of the wild Chinese shrimp in ten families which were built in different years.The male's genotypes could be determined from those of a female and her offspring at every locus.Only one combination of male genotypes was observed in each family.Farther,the sperm DNA of wild male was extracted from copulated wild female thelycum in three families of the ten.The result of microsatellite markers showed that the sperm DNA of wild males were not contaminated by female genome DNA.At each locus,every progeny had one allele found in the male genotype.The distributions of genotypes in offspring of the ten families were consistent with the Mendelian segregation.All of these suggested that one female copulated to one male and supported that the mating mode of this prawn is pair-mating.The cluster analysis based on UPMGA (unweighted pair-group method using arithmetic averages) between individual within/among families was also examined and showed that each family formed single cluster,respectively.Microsatellite markers as an effective tool for kinship analysis could be widely used to reveal the population structure,mating mode,etc.in breeding programs.