A PILOT STUDY ON ISOLATION OF MICROSATELLITE DNA IN THE YANGTZE FINLESS PORPOISE (NEOPHOCAENA PHOCAENOIDES ASIAEORIENTALIS)
长江江豚微卫星DNA分离的初步研究

To the aim of developing specific microsatellite markers for the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis,72 microsatellite DNA sequences were isolated using an improved FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) protocol.According to Weber's classification rules,these sequences were divided into three categories:perfect repeat sequences (58.3% of total),imperfect repeat sequences (22.2%),and compound repeat sequences (19.5%).Thirty PCR primer pairs were designed and synthesized,and then screened by a sample panel of 12 random selected Yangtze finless porpoises.As a result,14 of them were proved to be polymorphic exhibiting 2 to 13 alleles (mean 5.86 alleles/locus).Their average observed heterozygosity and expected heterozygosity were 0.560 and 0.709 respectively.These are the first batch of specific microsatellite markers for the Yangtze finless porpoise,and they should play great role in succedent conservation genetics studies.