Microsatellite markers simulation and application for parentage determination on fenneropenaeus Chinensis
中国对虾微卫星家系鉴定的模拟分析与应用

This study was initiated to assess the feasibility of parentage determination in aquaculture populations for Chinese shrimp (Fenneropenaeus chinensis). Under different scenarios both simulation and reality, microsatellite markers were used to assign parentage to offspring. Simulations based on allele frequency data from the population of Chinese shrimp demonstrated that at least 4 loci were required to assign over 95% of progeny. When marker data from five loci were combined, the assignment success of progeny to their true parental couple increased to 92.9% in parentage identification with known parental and filial information. Out of 215 offspring, 90.7% was assigned to their parental pairs exclusively in mixed families groups. This study showed that the pedigree of progeny in mixed families could be satisfactorily determined using as few as five microsatellite markers even though the shrimp could have originated from 30 possible pairs. Discrepancies between the simulations and real data sets were considered to be largely due to the mismatches caused by scoring errors at microsatellite loci. Analysis for the value of LOD would reduce the error occurred in parentage determination on Chinese shrimp. Based on these results, it was concluded that the use of a number of microsatellite markers represented a realistic and effective alternative to physical tagging in a selection program and it allowed the identification of parental effects on offspring performances from the early life stages.