THE EFFECT OF COLVENT POLARITY ON THERMODENATURATION OF CARBONIC ANHYDRASE
溶剂极性对碳酸酐酶热变性的影响

The effect of aqueous solution of alcohol with different length of hydrocarbon chain andconcentration on denaturation temperature and denaturation enthalpy of carbonic anhydrase was studied by differential scanning calorimetry, The results showed that the denaturationtemperature of carbonic anhydrase decreased as the concentration of methanol, ethanol andn-propyl alcohol increased respectively: the denaturation temperature decreased obviously asthe length of hydrocarbon chain of alcohol increased, the denaturation enthalpy of carbonicanhydiase in alcohol solution of lower concentration such as 10% was higher than that inpare buffer solution, but the denaturation enthalpy of carbonic anhydrase in alcohol solutionof higher concentration was lower than that in pare buffer solution and it decreased obviouslyas the length of hydrocarbon chain and the concentration of alcohol increased.