STUDY ON THE ALTERATIONS OF SOME PHYSICOCHEMICAL PROPERTIES IN HUMAN POLYMORPHOUNCLEAR LEUKOCYTE MEMBRANES BY ESTRA-, OR EXTRA- CELLULAR REACTIVE OXYGEN SPECIES
细胞内、外源活性氧致人多形核白细胞膜理化特性改变的研究

Some alterations of physicochemical properties in human polymorphonuclear leukocyte (PMN) membranes reacted by intra - or extra - cellular generated reactive oxygen species have been investigated by means of fluorescence labels 1, 6 -diphenyl - 1,3, 5hexatriane (DPH) and N - (3 - pyrene) maleimide (N (3p) M) No changes of the peak and shape of flurescence excitation and emission spectra was found, but obvious decrease in the fluorescence intensities and increase in the fluorescence polarizations of both labels in the membranes reacted by extra-cellular generated singlet oxygen (1O2) and superoxide anion (O2 ) were observed. All the above mentioned reactions could be relatively inhibited by L - histidine, superoxide dismutase (SOD) and catalase (CAT) The intracellular generated reactive oxygen species produced by stimulating PMN respiratory burst with opsonized zymosan give rise to the changes of fluorescence parameters of the labels in the reacted membrane being little different to the extracellular generated 1O2 and O2, and moreover the fluorescence intensity of DPH was obviously increased, but the scavenging actions of SOD and CAT on the reactive oxygen species were unlikely to be found.