STUDIES ON THE ADHESIVE MECHANICAL PROPERTIES BETWEEN HEPATOCELLULAR CARCINOMA CELLS AND ENDOTHELIAL CELLS
肝癌细胞与内皮细胞的粘附力学特性研究

The purpose of the study is to investigate the adhesive mechanical properties between hepatocellular carcinoma cells (HCC) in different cell cycle phase and human umbilical vein endothelial cells (HUVECs) by cellular biology and cellular mechanics, using micropipette aspiration and cell synchronization technique. It showed that the percentage of different phases of general HCC cells cycle are: G0/G1 phase 53.51%, G2/M phase 11.01%, S phase 35.48%; the synchronous G, and S phase HCC cells were achieved through thymine-2-desoxyriboside, colchicines sequential blockage method and double thymine-2-desoxyriboside blockage method, the synchronous rates of G1 and S phase amount to 69.02% and 96.50%, respectively. The adhesive forces changed with the variations of adhesive time and presented behavior characteristics of adhesion and de-adhesion; S phase SMMC-7721 cells have higher adhesion forces to HUVECs than G1 phase cells(P<0.01), so it can be suggested S phase cells may play main roles in its adhesion to endothelial cells.