KILLING EFFECTS OF PULSED ELECTRIC FIELDS ON MALIGNANT TUMOR CELL
陡脉冲电场对恶性肿瘤细胞杀伤效应的研究

In order to observe the lethal effects of pulsed electric fields (PEFs) of different parame-ter combinations (frequency, peak value of voltage, width of pulse) on malignant tumor cell, every PEF was applied to malignant tumor cell suspension (3 ml) in the test bottle for 20 min. The MTT method was used to test the mortality ratio of tumor cell and growth curve was plotted. The ultrastructural varia-tions of treated cells were inspected and compared with the untreated cells. The results showed that PEFs of different combinations had great influence on tumor cell. With the increasing of parameter value, the mortality ratio of tumor cell was rising and cell proliferation was inhibited. The treated cell was swollen and the membrane was destroyed. The mitochondria was also cavitated and swollen.