THE STUDY ON INITIAL TIME OF FLUORESCENCE RECOVERY IN THE FPR EXPERIMENT
在荧光漂白恢复测量中荧光恢复起始时间的研究

In fluorescence photobleaching recovery (FPR) , delay time exists between the first moment while the signal is sent out to close the bleaching shutter and the second moment while the shutter acts really. In order to protect the PMT from being damaged by the intensive light, the PMT shutter should wait for a moment to open (100-200ms) in general. The moment, when the bleaching is completed or the fuorescence recovery begins, couldn't be exactly determined. The fluorescence intensity at t=0 (i.e.f(0)) could not be detected accurately as well. These make the acquired data inaccurate in t-axis, and reduced the accuracy of result. For rapid diffusion, it becomes more serious.In this paper, we have estimated the errors arisen from the initial recovery time, and analysed the factors which affect the delay time of bleaching shutter. Futhermore, the delay time has been measured and the time error has been corrected. After appropriate selection of PMT waiting time as short as possible, the data are acquired more completely during the initial stage of fluorescence recovery, i,e.F(0) is more precise.