A NEW MODEL MEMBRANE AND ITS APPLICATION IN SKIN PENETRATION MECHANISM STUDIES OF AZONE
Azone类透皮剂作用机理及一种新型模型膜的研究

A new-designed egg membrane is proved to be a promising model in skin penetration studies. By use of 5-doxyl stearic acid as spin label for ESR spectra measurement, Azone as skin penetration enhancer and egg membrane incorporated with IPM and cholesterol as experimental model, the major penetration enhancement mechanism of Azone had been assessed. A decrease in order parameter of lipid in the presence of Azone is due to increased motion rate of long hydrocarbon chain both in lipids and 5-doxyl stearic acid. It indicates an increased fluidity of the lipids incorporated into egg membrane because of the effect of Azone, that provides evidance to support hypothes considering the intercelluar lipid to be a major pathway for percutaneous absorption of drugs.The results obtained were confirmed by the use of bare-mouse skin stratum as experimental model which may allow to model the conditions in vivo penetration.