Rapid Detection of Virulent Salmonella Strains Using Multiplex PCR

This study aims to establish a rapid and sensitive multiplex PCR Method for Salmonella detection and the differentiation of virulent strains. Four sets of primers that target hisJ, spvR and fliC were designed and the PCR reaction conditions were then optimized. Based on the detection of reference Salmonella strains and other reference bacterial genera, the PCR Method was able to detect hisJ, spvR, fliC-c and fliC-i in Salmonella. The minimum detectable DNA concentration of the method is 100 pg. The results using the reference strains were consistent with previous research. In addition, this method can also be used in identifying S. typhimurium and S. arizona. Researchers successfully developed a convenient and useful multiplex PCR Method for detecting Salmonella and monitoring virulent strains.