Characterization of PB-CS01, A Novel Photobacterium Strain Isolated from Commercial Pork

A Gram-negative, rod-shaped bacterial strain named PB-CS01 which is bioluminescence-positive was isolated from contaminated commercial pork that probably had been exposed to seafood. Optimal growth of strain PB-CS01 requires the presence of 3.0% (w/v) NaCl and a temperature of 20°C. Phylogenetic analysis based on 16S rRNA gene sequences of strain PB-CS01 and other Photobacterium species showed that the novel isolate belongs to the genus Photobacterium. Sequence similarity analysis between PB-CS01 and other species indicates that the closest relatives of strain PB-CS01 are Photobacterium phosphoreum ATCC 11040 (99.9%), Photobacterium kishitanii pjapo.1.1 (99.8%) and Photobacterium iliopiscarium ATCC 51760 (99.5%). The most abundant fatty acids were summed feature 3 (50.77%; C16:1ω7c and/or C16:1ω6c) and C16:0 (15%). The fatty acid profile is similar to that of the genus Photobacterium but this report is the first to describe C16:1ω6c as one of the compositions of summed feature 3 of the genus Photobacterium. The G+C content of the genomic DNA of strain PB-CS01 was 44.8 mol%. Overall, strain PB-CS01 is a novel Photobacterium species.