Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus

To develop a new, rapid, practical and sensitive method for detection of Porcine Epidemic Diarrhea Virus (PEDV), a Reverse Transcription Loop Mediated isothermal Amplification (RT-LAMP) assay was established based on six primers in the conserved gene. The method had specificity but no cross-reaction with other viruses and was 100 fold higher than RT-PCR. The result suggested that the newly developed RT-LAMP assay is a simple and specific method for rapid detection of PEDV in field conditions and it has a high practical value in PEDV integrated control and early diagnosis.