Histological Evaluation of Periodontal Regeneration Due to Using Collagen Membrane (Paroguide) in Surgical Defects of Sheep Teeth

This study evaluates the effect of paroguide membrane on periodontal regeneration process. The buccal surfaces of 2nd mandibular premolar of 4 sheep were selected. Then, a 5 mm deep defect was made from mesial to distal of tooth. Next, the shallow cementum of root was taken and the defect was covered with a sheet of paraguide membrane. After the flap was stitched, post operation and hygienic care were taken into consideration for 6 months. Finally, the sheep were slaughtered and the operated areas were examined histologically. The result indicated that APDL had occurred regularly and practically. No inflammation existed in the tested area. The mean bone regeneration was 60%. The generated bone was of Lamina type. Cementum formation was 4.01 mm consisting cellular and acellular cementum.