Pathogenecity Trial of IBV in Diffferent Route of Inoculation in Embryonated Chichken Eggs

Infectious Bronchitis Virus (IBV) which causes infectious bronchitis in poultry industry produces extensive economic loss to any poultry operation. Obvious failures in various vaccination programs necessitate the new vaccine development. Virus cultivation is the first step in vaccine production so, this study aimed in finding the best route of inoculation for virus cultivation in embryonated chicken eggs. The 5 and 9 days old embryonated chicken eggs were used and the inoculation of IBV is done in three routes namely yolk sac, chorio allantoic fluid and chorio allatoic membrane and the lesions were observed. More prominent observations like stunting embryo, Cashew nut appearance and expulsion of internal organs confirms that yolk sac route is the best route for cultivation of IBV.